Antimicrobial And Antifungal Activity On Glutathione Stabilized Silver Nanoparticles – An In-Vitro Study

I. V. Anambiga, V. Suganthan, N. Arunai Nambi Raj School of Biosciences and Technology, VIT University, Vellore, Tamilnadu, India School of Advanced Sciences, VIT University, Vellore, Tamilnadu, Indi [email protected], [email protected]@vit.ac.in Abstract: Silver nanoparticles were obtained by chemical reduction of silver nitrate in water with sodium borohydride (NaBH4) in the presence of glutathione (a tripeptide biomolecule) as a stabilizer. The synthesized silver nanoparticles were characterized by XRD, UV-Visble spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The antibacterial and antifungal activity of glutathione stabilized silver nanoparticles (GSH-AgNPs) was tested by using Gram-negative and Grampositive bacteria (Bacillus sp., E.coli, Streptococcus sp., Pseudomonas sp., and Rhizobium sp.) and fungal agents (Aspergillus niger and Candida albicans). The silver nanoparticles, whose bacterial activity was dependent on the aggregation degree between particles, exhibited bacterial activity against Bacillus sp. and similarly for fungal activity against C. albicans. The maximum zone of inhibition (mm) was shown by Bacillus sp. (gram negative bacteria) for antibacterial activity and for antifungal, maximum activity shown by C. albicans for 100μg of the sample.